A Digital PCR-Based Method for Efficient and Highly Specific Screening of Genome Edited Cells
نویسندگان
چکیده
منابع مشابه
A Digital PCR-Based Method for Efficient and Highly Specific Screening of Genome Edited Cells.
The rapid adoption of gene editing tools such as CRISPRs and TALENs for research and eventually therapeutics necessitates assays that can rapidly detect and quantitate the desired alterations. Currently, the most commonly used assay employs "mismatch nucleases" T7E1 or "Surveyor" that recognize and cleave heteroduplexed DNA amplicons containing mismatched base-pairs. However, this assay is pron...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2016
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0153901